- Lucía Barbier-Torres, Ben Murray, Jin Won Yang, Jiaohong Wang, Michitaka Matsuda, Aaron Robinson, Aleksandra Binek, Wei Fan, David Fernández-Ramos, Fernando Lopitz-Otsoa, Maria Luque-Urbano, Oscar Millet, Nirmala Mavila, Hui Peng, Komal Ramani, Roberta Gottlieb, Zhaoli Sun, Suthat Liangpunsakul, Ekihiro Seki, Jennifer E. Van Eyk, Jose M. Mato, Shelly C. Lu. Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease. Nature Communications, 2022; 13 (1) DOI: 10.1038/s41467-022-28201-2
The discovery, published in the peer-reviewed journal Nature Communications, can also help lead to a new treatment approach for people suffering from the disease.
Cases of alcohol-associated liver disease continue to rise and is one of the leading causes of alcohol-related deaths. The spectrum of the disease includes hepatitis, fibrosis to cirrhosis and liver cancer. Cirrhosis alone causes 1.6 million deaths worldwide and over 50% of cases are due to alcohol abuse. Besides abstinence, there currently are no effective therapies for treating people with the disease.
“Alcohol-associated liver disease is a major problem in the world,” said Shelly C. Lu, MD, director of the Karsh Division of Gastroenterology and Hepatology in the Department of Medicine and senior author of the study. “We’ve known for a long time that alcohol somehow damages mitochondria, but until now, it’s not been clear as to what the mechanisms are for this damage to occur.”
The liver is very rich in mitochondria, known as the powerhouse of all cells, and plays a critical role in liver function. Alcohol, however, can alter the structure and function of the mitochondria, leading to liver injury.
To better understand the mechanisms for mitochondrial damage in alcohol-associated liver disease, Lu and her team looked at the role of an enzyme called MAT?1 that’s responsible for providing the liver vital nutrients for survival.
Using liver tissues from patients with alcohol-associated liver disease and preclinical models, the team found levels of this enzyme were selectively reduced in the mitochondria.
“Once we saw the depletion of MAT?1, we needed to figure out what was making that happen,” said Lucia Barbier-Torres, PhD, a postdoctoral scientist in the Lu Laboratory and first author of the study.
The team found alcohol activates the casein kinase 2 (CK2) protein, which triggers a process called phosphorylation of MAT?1 at a specific amino acid residue. In their experiments, the team found this process facilitates an interaction between MAT?1 with another protein called PIN1 and prevents MAT?1 from transporting into the mitochondria.
“Once this interaction happens, MAT?1 cannot get into the mitochondria to provide the essential nutrient and instead gets degraded,” Barbier-Torres said.
With this information, the team decided to block this interaction by muting MAT?1, therefore preventing phosphorylation from occurring. This prevented the interaction of the two proteins, preserving mitochondrial MAT?1 location and function in the mitochondria and thus protected the mitochondria from being damaged by alcohol consumption. They observed the same protection when they reduced CK2 expression to lower MAT?1 phosphorylation.
“Our results support a novel and targetable mechanism to help treat alcohol-associated liver disease,” said Lu, who is also a professor of Medicine and the Women’s Guild Chair in Gastroenterology.
The next steps in this line of research for Lu and her team include developing small molecule therapeutics that can interfere with the interaction between MAT?1 and PIN1, which should protect the mitochondria from alcohol-mediated damage.
Additional Cedars-Sinai co-authors include Ben Murray, Jin Won Yang, Jiaohong Wang, Michitaka Matsuda, Wei Fan, Nirmala Mavila, Hui Peng, Komal Ramani, Ekihiro Seki and Jennifer Van Eyk.
Funding: The research was supported by the National Institutes of Health under award numbers R01AA026759 and R01DK123763.
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New study reveals potential target for alcohol-associated liver disease